Fluorescence-Activated Cell Sorting (FACS) of living cells separates a population of cells into subpopulations based on fluorescent labelling. Cells stained using fluorophore-conjugated antibodies can be separated based on fluorescent recognition using specialised lasers.
FACS can be used to carry out many functions including:
- High-speed cell sorting
- Apoptotic assays
- Cell cycle analysis
DNA analysis
The FACS Core Facility at AKU-CRM is equipped with the most advanced and state of the art cell sorters that include:
- BD FACSAria III SORP (Special Order Research Product), and
- BD FACSMelody
BD FACSAria III
BD FACSAria III can perform multi-parameter flow cytometric analysis with high throughput cell sorting. The equipment currently is capable of doing 9 colours and up to 4-way sorting. It is accompanied by BD FACSDiva software for analysis.
BD FACSMelody
BD FACSMelody can perform multi-parameter flow cytometric analysis and cell sorting. The equipment currently is capable of doing 6 colours and up to 4-way sorting. It is accompanied by BD FACSChorus and BD FACSDiva software for data acquisition and analysis respectively.
Service Fee
Services in this facility are provided by trained and dedicated staff on a fee for service basis. For more information and assistance, please contact:
Sadia Habib
Assistant Manager, Laboratory
